Chief Constable Tony Melville is to step down in protest at plans to bring in elected police commissioners.
Mr Melville says he has "grave concerns" about the new system and is leaving after just two years in the job.
The Chief Constable was recently outspoken about plans to freeze the police budget which he said would result in cuts.
Now he is stepping down two years after moving from Devon and Cornwall Constabulary.
The Chief Constable said he was leaving after 34 years in policing before being forced to work for an elected police commissioner.
He said: "I believe policing does need to change and that is why we have transformed our approach in Gloucestershire.
" However I have grave concerns about some elements of the current police reform agenda especially the election of Police and Crime Commissioners in six months time.
"I have therefore decided that I will not continue as Chief Constable under those new arrangements.
" I am stepping aside in time for the Police Authority to appoint my successor and ensure continuity for the incoming Police and Crime Commissioner in November"
So far ex detective Martin Surl is the only candidate for the Commissioners job which will be decided by an election November.
Mr is campaigning to keep Coleford police station open.
When Mr Melville leaves at the end of May, DCC Mick Matthews will be appointed temporary Chief Constable.
ACC Kevin Lambert will be appointed temporary Deputy Chief Constable into early 2013 when the situation will be reviewed with the new commissioner.
Police say that under the leadership of Tony Melville crime has fallen by 10%, confidence in the police has risen by 17% and the Constabulary has developed and nearly finished implementing large budget reductions.
Chair of Gloucestershire Police Authority, Councillor Rob Garnham, said members are sad to see him go.
He said: "We appointed Tony because we wanted, and felt he would be capable, of transforming the organisation – not just its structures, but also its culture and values.
"He has not disappointed. A successful programme of change is well underway within the organisation and a significant amount of positive transformation has already taken place.
"Whilst we would have wished him to stay, the timing allows us to plan for his succession so that this important work can continue. We wish Tony all the very best for his future."





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